Remove the plate sealer and read on an HTRF® compatible reader . On BMG LABTECH´s NEPHELOstar Plus, a laser diode (at 635 nm) with a highly collimated beam is used as high-intensity light source. For more information. For more information about HTRF. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. Certified for use with Alpha, LANCE ® or DELFIA ®. Microplate reader assays requiring infrared (IR) or. The emission of light by the acceptor will be proportional to the level of interaction. Cells in each image are identified by SoftMax Pro Software and cell-by-cellHTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. Place the imaging filter cube on top of the LED cube you installed, and use the hex wrench to screw it to the LED cube. no. For more information about HTRF ®Seal the plate and incubate for Overnight. 1. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. Table 1. To perform the assay, use white plate only. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. Suggested plate map: 1: 2 3: A: 14 μL Std 0: 3 μL IP1 d2 Reagent 3 μL IP1 Tb Cryptate AntibodyCisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. At Cisbio we provide microplates that help you discover better. Lysates are then transferred to the assay plate for the detection of Total-eNOS by HTRF. This protocol, HTS designed, allows miniaturization while. If not, our scientific team will help you choose the instrument that best suits your needs and budget. Microplate Readers and Reader Accessories. The mean and standard deviation. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Plate Reader Requirement. Experimental details• HTRF®-Certified Reader **. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. This protocol gives the cells viability and confluence to be monitored. (665 nm and 620 nm) on a compatible HTRF ® reader. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Add ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. FOR HTRF CERTIFIED READER. 5 μL of Binding buffer 2X. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. Suggested plate map: 1: 2 3: A: 14 μL Std 0: 3 μL IP1 d2 Reagent 3 μL IP1 Tb Cryptate Antibody• HTRF®-Certified Reader. HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. For HTRF certified readerThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Make sure the setup for Tb Cryptate is used. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. For more information about microplate recommendations, please visit our website • Store the kit at -16°C. The plate is set up for reading and images are acquired according to specified parameters. This protocol enables the cells' viability and confluence to be monitored. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. FOR HTRF CERTIFIED READER. HTRF is compatible with a large range of available microplate readers. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. This protocol gives the cells viability and confluence to be monitored. Plate 25 μL of cells in. 14 seconds for a 384-well plate. FOR HTRF CERTIFIED READER. Tecan Spark multimode microplate reader, with fluorescence Fusion Optics™ meets these challenges and offers everything you need to develop and run advanced biochemical and cell-based fluorescence assays. EGFR protein (PV3872, 0. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for excellence. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. After defining the objectives and their locations in Gen5: 1. Make sure the setup for Tb Cryptate is used. White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. For a list of HTRF-compatible readers and set-up recommendations, please visit . If not, our scientific team will help you choose the instrument that best suits your needs and budget. 9. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Unlike fluorescence intensity which focuses on the quantification of emission intensity at a specific wavelength and neglects its polarization, fluorescence polarization specifically analyses as output the emission intensity of. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. See the Cytation 5 Operator’s Manual for more information and instructions. The EnSight<sup>®</sup> multimode plate reader offers HTS Alpha and Ultra-sensitive Luminescence technologies alongside conventional multimode detection technologies. Make sure the setup for Eu 3+ Cryptate is used. No washing steps are required. Read & analyse. COMPONENTS ; STORAGE . This protocol enables the cells' viability and confluence. Make sure the setup for Tb Cryptate is used. • ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. STANDARD . HTRF Microplate Readers. Incubate overnight at RT. Plate 25 μL of cells in. * For HTRF microplate recommendations, please visit binding of the fluorescent ligand to the receptor, TR-FRET occurs and is measured with an HTRF-certified microplate reader. For a list of HTRF-compatible readers and set -up recommendations, please visit. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. Figure 1. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm:. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. If not, our scientific team will help you choose the instrument that best suits your needs and budget. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes To prevent evaporation during assay incubation steps, we recommend the use of TopSeal-A PLUS, a clear adhesive seal for microplate. The Z´ value takes into account four (4) parameters: the means (µ) and the standard deviations (σ) of the positive (plateau) and the negative (baseline) controls 1. For more information about HTRF. The multidetection microplate readers CLARIOstar, PHERAstar FS, FLUOstar Omega, and POLARstar Omega are all HTRF-certified by Cisbio, meaning each of the. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. These are the different kinetic modes on BMG LABTECH microplate readers. Please note that this kit use a Terbium donor crypate, make sure to set up your reader for Terbium cryptate. It can also be further streamlined toLysates are then transferred ®to the assay plate for the detection of Total TYK2 by HTRF reagents. HTRF Signal + 5 hours @ RT or . FOR HTRF CERTIFIED READER For more information about HTRF. Fluorescence intensity – Fluorescence intensity is one of the most. FOR HTRF CERTIFIED READER. High Speed Monochromator offers a typical wavelength accuracy of <0. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. 96-well culture plateHowever, time-resolved fluorescence (TRF & TR-FRET) assays are used in conjunction with fluorophores that have a longer half-life. ® Detection of Progesterone Receptor with HTRF reagents is performed in a single plate used for plating, stimulation and detection. FOR HTRF CERTIFIED READER. MATERIALS PROVIDED: KIT . Plate 25 μL of cells in 96 half-well plate in your appropriate medium. For more information about HTRF ®• HTRF®-Certified Reader. culture plate. Lysates are then transferred to the assay plate for the detection of phospho-Tie2 (Tyr992) by HTRF. For more information about microplate recommendations, please visit our website • Store the kit at -16°C. $352. Do not pre-mix the d2 and Cryptate solutions prior to dispensing Make sure to use the set-up for Eu Cryptate. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. BMG LABTECH’s UV-laser not only allows for faster read times in europium TRF assays, but it also greatly enhances performance in terbium-based TRF assays. Lysates are then transferred to the assay plate for the detection of total-p53 by HTRF. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. The ratio for each well must be calculated individually. HTRF plate reader. Incubate 2 h at RT. Some vendors hold the entire market share for not only a. The DLR assay is a two step reaction that uses two luciferase enzymes, Firefly and Renilla (Figure 1). Read other about this measurement approach here. For more information about HTRF ®Excitation range. culture plate. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. They can be used in a no-transfer. Example assays are ELISAs, DNA and protein concentration determination, steady-state dose response curves, ADP-Glo™ (Promega), Transcreener™ GDP fluorescent polarization. FOR HTRF CERTIFIED READER. The Negative control is used to check the non-specific signal. The PHERAstar ® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. This protocol gives the cells viability and confluence to be monitored. The VICTOR Nivo is a HTRF certified microplate reader. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. Seal the plate and incubate 1 hour at RT : Remove the plate sealer and read on an HTRF® compatible reader . Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. certified for use with HTRF. FOR HTRF CERTIFIED. FOR HTRF CERTIFIED. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. FOR HTRF CERTIFIED READER. Lysates are then transferred to the assay plate for the detection of Total Cofilin by HTRF® reagents. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-2 (Ser473) by HTRF. It utilises an ultra-fast UV/vis spectrometer or filters for absorbance as well as highly sensitive filters for all other detection modes. Conclusion. Figure 1: Principle of HTRF Cortisol competitive assay. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. MUTANT HTT STANDARD WORKING SOLUTIONSLysis buffer 4X for two-plate assay protocol on suspension cells Determine the amount of lysis buffer needed for the experiment. For more ® information about HTRF compatible readers and for set-up recommendations. HTRF Setup. FOR HTRF CERTIFIED READER. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. For more. n HTRF plate validated (QC with reference HTRF assays) n Low to high volumes (10 µL to 350 µL) n Can be used with all HTRF compatible readers n For cell-based or biochemical assays Trying is Believing! Plate Characteristics HTRF® 96 - well low volume plate CulturPlateTM 96 - well Microplate CulturPlate TM 384 - well Microplate ProxiPlateTM 384 - Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERDetection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. The laser beam passes through the sample well. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. For more information about HTRF ®Highly compatible plates for HTRF. Make sure the setup for Eu3+ Cryptate is used. Part Number# Product Description Number of Plates# 66PL96001 HTRF 96 well low volume plate 1 plate 66PL96005 HTRF 96 well low volume plate 5 platesThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf certifiedLysates are then transferred ®to the assay plate for the detection of Total CHK2 by HTRF reagents. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non-specific short-lived emissions. $1,024. Results for HTRF Reader Control Kit with black and white microplates. Bench top solutions for cell based assays. Its unique optical design and instant polarizer switching provide the smallest mP standard deviation in any assay. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. An. Taking these considerations into account, Cisbio’s 96w low volume and PKI’s 96w & 384w microplates have been carefully designed to provide the best signal detection environment possible to all HTRF assays. Lysates are ®then transferred to the assay plate for the detection of phospho- Rab10 (Thr73) by HTRF reagents. 96-well or 384-well small volume (SV) detection microplates - For more information about microplate recommendations, please. PURCHASE SEPARATELY . EnVision Nexus ultimode Plate Reader 2 Benefits • Provides the speed and sensitivity to boost your lab’s productivity with ultra-high throughput • Freely configurable and upgradable to adapt to your needs today and tomorrow • Fully automatable for walkaway convenience • Certified for use with HTRF® technology Key featuresBMG LABTECH plate readers certified for LanthaScreen detection include the PHERAstar FSX, CLARIOstar Plus and VANTAstar. EX: HTRF Emission range. One plate carrier adapter is provided with the instrument. On the SpectraMax i3x reader, install the HTRF cartridge, and wavelengths will be selected automatically when HTRF is selected as the Optical Configuration. Reader. Make sure the setup for Tb Cryptate is used. It fits any benchtop – and budget Building on our pioneering family of benchtop multimode plate readers, the VICTOR® Nivo™ multimode reader breaks new ground, packing all popular detection modes into the industry’s smallest benchtop. HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed TNFα Antibodies ADD READ ANALYSE Incubate 2 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. Add. The VICTOR Nivo. Make sure the setup for Eu3+ Cryptate is used. Read & analyse. It can also be further streamlined toFOR HTRF CERTIFIED READER. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. Incubate 4h at RT. PROTOCOL AT A GLANCE Do not pre-mix the d2 and Cryptate solutions prior to dispensing. When equipped with a quad monochromator, the EnSight plate reader can improve performance for absorbance and fluorescence detection. It can also be further streamlined to a one-. 0. Incubate at room temperature. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Plate Type and Read Area should be selected based on the microplate used and wells to be read. Preconfigured HTRF protocols in SoftMax ® Pro Software 7. 1) . FOR HTRF CERTIFIED READER For more information about HTRF. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. 6% for 1,408 wells and a Z’ value of 0. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. It enables you to gain insights you couldn’t achieve with detection measurements alone. We would like to show you a description here but the site won’t allow us. HTRF-compatible SpectraMax Multi-Mode Readers. How HTRF can simplify your life The PHERAstar FSX is the best microplate reader for all HTRF assays: Sensitive, fast and reliable measurements with best Z´ and DeltaF% HTRF-certified with white and black plates; Dedicated excitation laser and detectors (PMTs) for best sensitivity; Simultaneous detection of donor and acceptor gives speed and precision See full list on bmglabtech. These plates can be read on all HTRF certified readers, thus making their implementation in. Plate 25 μL of cells in. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the market with all major detection technologies including Alpha and certification for HTRF® technology plus software designed for 21 CFR Part 11. . The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of Total Cereblon HTRF detection reagents. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. * For HTRF microplate recommendations, please visit . Plate 25 μL of cells in. 5. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Make sure to use the set-up for Eu Cryptate. Lysates are then transferred to the assay plate for the detection of phospho-c-Src (Tyr419) by HTRF. It is used as a positive control for Gs applications ( biological models validation,Detection of Total CDK12 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. The Dual-Luciferase ® Reporter Assay or DLR is widely used to study gene transcription and regulation. 5 nm filter (PerkinElmer 2100-5110) were used. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. Each well generally requires 50µL of lysis buffer 1X. Optimized instrument settings for the HTRF camps HiRange assay. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. In particular, these parameters should be entered as below. Plates can be loaded manually or plates can be loaded using 20 or 50-plate free access magazines which fit in a stacker. For HTRF certified reader For more information about HTRF® compatible readers and for set- up recommendations, please visit our website. The lysis buffer 4X is ready-to-use. • HTRF®-Certified Reader. Using a black. PURCHASE SEPARATELY . CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. First the plate is coated with a capture antibody. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. No washing steps are required. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. DATA REDUCTION & INTERPRETATION 1. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. FOR HTRF CERTIFIED. This protocol gives the cells viability and confluence to be monitored. * For HTRF microplate recommendations, please visit . Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. We recommend using a white plate for time-resolved fluorescence assays and other assays where a lower signal is to be expected, to reduce the signal from background autofluorescence. Lyse cells Read on an HTRF compatible reader . DLReady™ certified luminometer plate readers. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. 5 EXAMPLE OF MAP PLATE . After an overnight incubation, the plate is washed 3 times. 45mm 5000µs Delay time Reading time Operation mode 100 µs 400 µs by plate 100 µs 400 µs by plate • This reader only allows high performance HTRF measurement when assays are run in WHITE plates. • Select Excitation Filter 320x40 HTRF Eu cryptate* • Select Excitation Optic Empty • Select Emission Filter 495xm10uv • Select Emission Optic 2 – FPThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. FOR HTRF CERTIFIED READER. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option. Read & analyse. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate 25 µL cells + 5 µL compounds + 10 µL supplemented lysis buffer (4X)* + 4 µL HTRF® pre-mixed antibodies Read & analyse Incubate 4h at RT Transfer 16 µL supernatant LysisAdd ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified . The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. PLATE READER INTRODUCTION Selecting the right plate reader for your lab can be challenging. As shown in Fig. Neocarzinostatin dose-response on Jurkat cells. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. For HTRF readings, a 620/40 nm filter (PerkinElmer 2100-5760) and a 665/7. Incubate overnight at RT. FOR HTRF CERTIFIED READER. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. For the standard curve described, all wells of the microplate are read. HTRF reagent concentrations have been set for optimal assay performances. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. 3. It combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option. CLARIOstar is equipped with a specific optical device which enables the measurement of. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes• HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. Better sensitivity provides better statistic, less variability among replicates, and a larger assay window. For more information about HTRF ® compatible readers and for set-up recommendations, please. The reader only allows high performance for the used HTRF measurement when the HTRF is run with white plates. No washing steps are required. With this certification, Cisbio Bioassays guarantees that the Synergy H4 meets or exceeds strict performance and functional criteria for optimal HTRF® readout. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The 2 plate protocol involves culturing cells in a 96-well plate before lysis then transferring lysates to a 384-well low volume detection plate before adding Phospho-ERK (Thr202/Tyr204) HTRF detection reagents. Lysates are then transferred to the assay plate for the detection of total-Rb by HTRF. pre+ 5 µL HTRFcompounds Activation Buffer. + 4 µL HTRF ® pre-mixed antibo dies 2. TO PREPARE TISSUE SAMPLES: Please refer to the Technical Note “Optimize Your HTRF® Cell Signaling Assays on Tissues” onHow to use Synergy microplate readers for HTRF detection, a versatile technology for drug discovery and life science research. FOR HTRF CERTIFIED READER. It measures at any wavelength, with top and bottom reading for most standard technologies. Plus . Your lab might even have one already. and Canaa -8-963-4567. Alpha Technology: The VICTOR Nivo system. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. FOR HTRF CERTIFIED READER. Read & analyse. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. -Certified Reader** *Assay volumes can be adjusted proportionally to run the assay in 96- or 1536-well microplates. Dedicated UV-laser excitation. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. It accepts all types of microtitration plate. It features a modular design that allows users to add or change detection cartridges according to their needs. The plate reader. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. Read & analyse. Discover the features and benefits of the VICTOR Nivo multimode plate reader, the smallest and most versatile instrument for life science applications. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYDetection. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. This DLReady-certified microplate reader can also come equipped with Simultaneous Dual Emission for BRET applications and can be further upgraded to a multi-mode reader. For more information about HTRF. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. Assay setup for a 384-well low-volume plate. But sometimes these parameters are not sufficient enough. Read & analyse. Each well requires 10 μL of 4X lysis buffer /well. CulturPlate-96: White Opaque 96-well Microplate, Sterile and Tissue Culture Treated. Make sure the setup for Tb Cryptate is used. Plate 25 μL of cells in. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. NC1286225. Up to 8 decades dynamic range in one measurement with EDR technology. Data Analysis HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same . The VICTOR Nivo is a HTRF certified microplate reader. Assay plates were incubated at RT and read at different time points thereafter on an HTRF ® certified. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Thanks to the unique Optic Module system, Simultaneous Dual Emission, UV/vis spectrometer, AAS System, and dedicated AlphaScreen ® and. FOR HTRF CERTIFIED READER. The PHERAstar FSX is the best microplate reader for all HTRF assays: Sensitive, fast and reliable measurements with best Z´ and DeltaF% HTRF-certified with white and black. Incubate 4h at RT. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Handle complex biological matrices easily. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Learn about the principles, applications, and benefits of HTRF in this Agilent application note. Cell seeding densities of 2,000,000 cells/well are generally sufficient for most cell lines, but. The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates to a low volume detection plate (either HTRF 384-lv or 96-lv plate) before the addition of HTRF Phospho-Rb (Ser780) detection reagents. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. (donor) of compounds are measured on an HTRF-compatible reader. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED. Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates. HTRF certified . You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. 47 seconds for a 1536-well plate. -CERTIFIED READER IS NEEDED. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. For more information. HTRF is compatible with a large range of available microplate readers. protocol with suspension cells or with a. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. FOR HTRF CERTIFIED. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. HTRF Signal + 16 µL 4 µL Standard of pre-mixed IL8 Antibodies or Sample ADD READ ANALYSE Incubate 1 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. FOR HTRF CERTIFIED READER For more. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. For more information about set-up and compatible HTRF ® readers,• HTRF®-Certified Reader. 5 µL. In this case the Z´(pronounced Z prime) value comes to mind. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. These plates can be read on all HTRF certified readers, thus making their implementation in. For more information. The assay was prepared in both white and black plates and incubated >18 hours. The donor and acceptor can be used to label a variety of biomolecules, for applications.